Table 1.

Lifetimes of AT₁R-EGFP in AT₁R-HEK293 cells. Cells were treated with Ang II (100 nM) at the indicated time periods and fixed, as described in Section 2. N, number of cells analyzed; data are mean±S.E.; ANOVA, Student–Newman–Keuls test. No significant differences among the control groups were detected.

Donor	Acceptor	N	Pretreatment	Ang II (min)	AT1R lifetime τm (ns)
AT1R- EGFP	None	6	None	0	2.33±0.07
		4		5	2.33±0.11
		5		30	2.31±0.08
AT1R- EGFP	Alexa 555 alone	5	None	0	2.33±0.04
		4		5	2.31±0.11
		5		30	2.32±0.28
EGFP alone	None	6	None	0	2.56±0.30
		5		5	2.56±0.27
		6		30	2.57±0.33
EGFP alone	Alexa 555 alone	5	None	0	2.61±0.41
		7		5	2.59±0.35
		7		30	2.53±0.19