Figure 2.

Deletions at the Cryge promoter. (a) Deletion constructs. Schematic overview of the deletion constructs of the Cryge promoter cloned into the reporter gene CAT or Luc. Putative binding sites for Pax6 and Prospero are given in black. CCR is the γ-crystallin common region (21), including the Cryner, Silencer, Crygpel and γF-1 elements as well as the Sox1- and γFBP-binding sites, as described in Figure 1. (b) Effects of deletion on the Cryge promoter. Transfection experiments were performed with N/N1003A or αTN4 lens epithelial cells or fibroblast-like NIH 3T3 cells. In all transfections a CAT construct was co-transfected with pCMVβ vector for internal standarisation of efficiency of gene transfer. Cell extracts were measured for CAT expression by CAT ELISA and for β-galactosidase activity. All data were normalised to the promoter-less plasmid, which was set as 1.