Figure 5.

Altered Prox1 activation of mutated γF-crystallin promoters. The left control shows γF-crystallin promoter activity, which was co-transfected with the pcDNA3.1 expression vector not containing PROX1. In the right control, 29 of 36 point mutated γF-crystallin promoters are activated by PROX1, on average to 10-fold of their basal activities. On transfecting five of 36 mutated plasmids (7, 38, 29, 15 and 36) Prox1 produced no or reduced activation of the promoters as compared to their basal activity. In transfection experiments of two (30 and 31) of 36 mutated plasmids a 30-fold activation was observed. All mutations are represented in Figure 10.