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. 2001 Jan 15;29(2):515–526. doi: 10.1093/nar/29.2.515

Figure 7.

Figure 7

Figure 7

Six3 represses the Crygf promoter. (a) Increasing amounts of Six3 were co-transfected into CD5A and N/N1003A cells. Six3 represses the γF-cyrstallin promoter to <20% of its basal activity. To transfect a constant amount of DNA the increasing amount of Six3-pcDNA3.-1 was compensated for by decreasing the amount of the empty vector pcDNA3.-1. At the origin of the graph only pcDNA3.-1 was used. In control experiments using a reporter plasmid without the Crygf promoter and with Six3 overexpression in CD5A cells no alteration in relative luciferase activity was observed. (b) Constant amounts of the pγ226LucII (2 µg) and PROX1-pcDNA3 (300 ng) plasmids were co-transfected into N/N1003A cells together with different amounts of Six3-pcDNA3.1 (0–90 ng). 100% is the basal activity of the untreated Crygf promoter. Six3 is able to obliterate Prox1 activation.