Figure 9.

Reduced Six3 repression in mutated γF-crystallin promoters. The left control shows γF-crystallin promoter activity when co-transfected with the pcDNA3.1 expression vector not containing Six3. In the right control, 28 of 36 point mutated γF-crystallin promoters are repressed, on average to 20% of their basal activities, due to co-expression of Six3-pcDNA3.1. Transfecting eight of 36 mutated plasmids (12, 1, 30, 3, 2, 38, 37 and 9) Six3 produced diminished repression of the promoters to between 40 and 100% of their basal activities. All mutations are represented in Figure 10.