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. Author manuscript; available in PMC: 2011 Nov 22.
Published in final edited form as: Carbohydr Res. 2010 Sep 18;345(17):2499–2506. doi: 10.1016/j.carres.2010.09.013

Table 1. Rates of bovine heart cytochrome c reduction under various conditions at 37 °C, pH 7.5.

[Lys]a
(mM)		 [R5P]a
(mM)		 [Cyto-chrome c]a
(μM)		 Additivea
(mM)		 Preincubation of Amine and R5P? Cytochrome c Reduction Rate
(μM min-1)		 O2- Generation Rateb
(μM min-1)
0 10 82 None --- 0.42 0.20
100 10 82 None None 1.8 1.10
100 10 82 None 0.5 h 3.0 0.85
0 10 82 Catalase (94 U/mL) None 0.60
0 0 82 2,3-BD (10) None 0 0
100 0 82 2,3-BD (10) None 1.2 ---
100 0 82 2,3-BD (10) 0.5 h 2.4 0.9
100 0 82 2,3-BD (10) 0.5 h 2.4 ---
ATP (10)
0 10 82 ATP (10) None 0.22 ---
100 10 82 w/o ATP 0.5 h 2.5 ---
with ATP (10) 0.5 h 2.5 2.5
0 10 82c None None 0.52 ---
0 10 82c ATP (10) None 0.24 ---
a

Concentrations of reagents listed are those when incubated with cytochrome c.

b

O2- generation rate was determined by the percent decrease in cytochrome c reduction rate upon addition of SOD (76 U/mL) to the system.

c

Yeast cytochrome c (from Sigma–Aldrich Chemical Co.)