Table 2. Rate constants for cytochrome c reduction by various sugars.
Sugar | Rate Constanta (mM-0.5 min-1) |
---|---|
R5P | 5.0 × 10-2 |
Ribose | 1.4 × 10-2 |
Ribose and Pi | 1.6 × 10-2 |
Glucose 6-Phosphate | 0.65 × 10-2 |
Fructose 6-Phosphate | 1.6 × 10-2 |
Glucose | 0.44 × 10-2 |
Glucose and Pi | 0.37 × 10-2 |
Arabinose 5-Phosphate | 3.7 × 10-2 |
2-Deoxyribose 5-phosphate | 0 |
R5P with ribose 5-phosphate isomerase (12 kU/mL)b | 25.0 × 10-2 |
All rate constants calculated as first order in respect to cytochrome c concentration and half order in respect to sugar concentration.
Rate constant calculated based on an equilibrium amount of 30% Ru5P (in comparison to R5P