Figure 3.
Expression of genes involved in metamorphic commitment during Ae.aegypti fourth instar development and in response to different feeding regimens. Representative images of RT-PCR products obtained for one experimental cohort are shown. H, head; T, thorax; A, abdomen. See Table 1 for gene identification and primers. See Figure 1 for image of actin loading control. AeaBrCZ4, AeaCPF1-2, and AeaCPR-7 showed differential expression in response to fourth instar feeding.