Figure 2. Mir155^−/− mice exhibit defective inflammatory T cell development during EAE.

Mir155^+/+ and Mir155^−/− mice were harvested 25 days following immunization with MOG_35–55. A. Intracellular staining was conducted to identify total lymph node cells (top) and CD4⁺ lymphocytes (bottom) producing IL-17A and/or IFN-γ (n=4). B. Splenocytes were analyzed as in (A) (n=4). C. Mir155^+/+ or miR155^−/− splenocytes harvested from mice 25 days after EAE induction were labeled with CFSE. CFSE loss by CD4⁺ proliferating cells from both groups was assayed by flow cytometry following restimulation with MOG_35–55 (20 µg/ml) for 72 hours (n=4). D. ³[H] thymidine incorporation was also assays using replicate cultures (n=4). 2 naïve WT mice were also included as controls. E. Production of IL-17A and IFN-γ by cells from (C.) was determined by ELISA (n=4). Data represent two independent experiments. Error bars represent +/−SEM and * denotes statistical significance with a p value of <0.05 according to a student’s two-tailed t-test. +/+ = Mir155^+/+; −/− = Mir155^−/−. See also Figure S2.