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. Author manuscript; available in PMC: 2011 Oct 29.
Published in final edited form as: Immunity. 2010 Sep 30;33(4):607–619. doi: 10.1016/j.immuni.2010.09.009

Figure 5. miR-155 expression by CD4+ T cells is necessary for proper Th17 cell development in vitro.

Figure 5

A. CD4+ T cells were isolated from Mir155+/+ or Mir155−/− spleens and cultured in the presence of plate bound CD3 and soluble CD28 antibodies, with (Th17 cell) and without (Th0 cell) IL-6 (50 ng/ml) and TGF-β (2 ng/ml). After 96 hours, expression of IL-17A and IFN-γ was assayed by intracellular staining following by flow cytometry. B. Results from a representative experiment are represented graphically (n=2). Data represent three independent experiments. C. Expression of Mir155 was measured by qPCR before and after activation with CD3 and CD28 antibodies (n=3). D. Expression of BIC, Mir155, and IL-17A in WT and Mir155−/− CD4+ T cells was assayed by qPCR following 96 hours of culture with CD3 and CD28 antibodies alone, or in Th17 cell skewing conditions (n=2). Data represent two independent experiments. Error bars represent +/−SEM.