Table 6.
Comparison of literature values for β-cell content in human islets
| Volume Fractionβ cells in Whole Islets | Number Fraction β Cells Amongst Islet Cells | |||
|---|---|---|---|---|
| Tissue Examined | Method | ΦβI | fβIC | Reference |
| Freshly Isolated Islets | EM Morphology | 0.61 | 0.74 | This study |
| Cultured Islets | IHC-LSC Microscopy | 0.541 | 0.65 | Brissova et al. (16) |
| Cultured Islets | IHC | 0.572 | 0.68 | Keymeulen et al. (5) |
| Dissociated cells | IHC-LC Cytometry | 0.43 | 0.51 | Ichii et al. (17) |
| Dissociated cells | IHC | 0.47 | 0.57 | Street et al. (3) |
| Pancreatic block | IHC-LSC Microscopy | 0.46 | 0.553 | Cabrera et al. (15) |
| Pancreatic block | IHC | 0.524 | 0.62 | Butler et al. (12) |
| Pancreatic block | IHC | 0.595 | 0.71 | Yoon et al. (11) |
| Pancreatic block | IHC | 0.636 | 0.75 | Sakuraba (14) |
| Pancreatic block | IHC | 0.68 | 0.80 | Kloppel et al. (10) |
| Pancreatic block | IHC | 0.65 | 0.78 | Kloppel et al. (13) |
| Pancreatic block | IHC | 0.70 | 0.73 | Rahier et al. (27) |
| Pancreatic block | IHC | 0.757 | 0.90 | Stefan et al. (28) |
| Pancreatic block | IHC | 0.637 | 0.76 | Malaisse-Lagae et al. (7) |
IHC = Immunohistochemistry with standard fluorescence microscopy unless otherwise noted; LS = Laser scanning; LSC = Laser scanning confocal
Underlined number is data as reported or after conversion of data to ΦβI or fβIC from equations in Appendix. Companion number is calculated using Equation (10).
Islets shipped and cultured for 48 hr total.
Islets cultured 7-14 days under conditions favoring β-cell enrichment.
Only cells that had a clearly labeled nucleus were counted.
Lean, nondiabetic subjects.
Korean subjects
Japanese subjects
Volume-weighted calculated value for 90% glucagon-rich, PP-poor lobes, 10% PP-rich lobes. All other studies with pancreatic blocks did not include PP-rich lobes.