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. 2001 Jan 15;29(2):373–379. doi: 10.1093/nar/29.2.373

Figure 4.

Figure 4

Figure 4

Variation of RPA concentration does not influence RPA crosslinking by 5′-end photoreagents. (A) Photoreagent V and (B) photoreagent VII were used for crosslinking experiments. Reaction mixtures were composed as described in Materials and Methods. After complete primer elongation RPA was added to the reaction mixtures to the indicated (bottom) concentrations followed by UV irradiation. The crosslinked products were separated by SDS–PAGE and visualized by autoradiography. The positions of crosslinked products and protein markers are indicated in the left and right margins, respectively.