Table 2. Tissue factor and HER2/neu expression in primary USPC cell lines.
| HER2/neu IHC | HER2/neu FISH | Tissue factor RT–PCR |
Tissue factor flow cytometry
|
||
|---|---|---|---|---|---|
| Sample | Cell block | Cell block | mRNA copy number | % Gated | MFI |
| Control | — | — | 1 | — | — |
| USPC-ARK-1 | 3+ | 2.5 | 14.6 | 86.7 | 32.7 |
| USPC-ARK-2 | 3+ | 5.2 | 332.3 | 100.0 | 92.2 |
| USPC-ARK-3 | 3+ | 4.7 | 280.2 | 100.0 | 89.8 |
| USPC-ARK-4 | 0 | 1.6 | 4.7 | 75.6 | 25.8 |
| USPC-ARK-5 | 0 | 1.4 | 17.6 | 55.3 | 53.6 |
| USPC-ARK-6 | 1+ | 0.9 | 816.4 | 100.0 | 92.5 |
Abbreviations: FISH=fluorescence in-situ hybridization; IHC=immunohistochemistry; MFI=mean fluorescence intensity; RT–PCR=real-time PCR; USPC=uterine serous papillary adenocarcinoma.