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. 2010 Jul 13;103(6):910–917. doi: 10.1038/sj.bjc.6605790

Table 3. Sequences of primers used in real-time RT-PCR analysis.

  Assay type Primers
Human PCR assays
sFRP1 (NM_003012) TaqMan F, 5′-CCAATCCCACCGAAGCCT-3′
    R, 5′-ATGATGGCCTCAGATTTCAACTC-3′
    P, 5′-CAAGCCCCAAGGCACAACGGTG-3′
NKD1 (NM_033119) TaqMan F, 5′-TCGCCGGGATAGAAAACTACA-3′
    R, 5′-CAGTTCTGACTTCTGGGCCAC-3′
    P, 5′-CCAATTTGGGCCTGGCTCCCC-3′
FZD3 (NM_017412) TaqMan F, 5′-CATGGAGATGTTTGGTGTTCCTT-3′
    R, 5′-AAGTCGAGGATATGGCTCATCAC-3′
    P, 5′-TCTGGGAACCTACTGCATTCCATATCTTCAGG-3′
c-myc (NM_002467) TaqMan F, 5′-AGCTGCTTAGACGCTGGATTTT-3′
    R, 5′-TTCCTGTTGGTGAAGCTAACGTT-3′
    P, 5′-CAGCCTCCCGCGACGATGC-3′
KRT8 (NM_002273) TaqMan F, 5′-GATCGCCACCTACAGGAAGCT-3′
    R, 5′-ACTCATGTTCTGCATCCCAGACT-3′
    P, 5′-CCGGCTCTCCTCGCCCTCCA-3′