Human PCR assays
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sFRP1 (NM_003012) |
TaqMan |
F, 5′-CCAATCCCACCGAAGCCT-3′ |
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R, 5′-ATGATGGCCTCAGATTTCAACTC-3′ |
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P, 5′-CAAGCCCCAAGGCACAACGGTG-3′ |
NKD1 (NM_033119) |
TaqMan |
F, 5′-TCGCCGGGATAGAAAACTACA-3′ |
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R, 5′-CAGTTCTGACTTCTGGGCCAC-3′ |
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P, 5′-CCAATTTGGGCCTGGCTCCCC-3′ |
FZD3 (NM_017412) |
TaqMan |
F, 5′-CATGGAGATGTTTGGTGTTCCTT-3′ |
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R, 5′-AAGTCGAGGATATGGCTCATCAC-3′ |
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P, 5′-TCTGGGAACCTACTGCATTCCATATCTTCAGG-3′ |
c-myc (NM_002467) |
TaqMan |
F, 5′-AGCTGCTTAGACGCTGGATTTT-3′ |
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R, 5′-TTCCTGTTGGTGAAGCTAACGTT-3′ |
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P, 5′-CAGCCTCCCGCGACGATGC-3′ |
KRT8 (NM_002273) |
TaqMan |
F, 5′-GATCGCCACCTACAGGAAGCT-3′ |
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R, 5′-ACTCATGTTCTGCATCCCAGACT-3′ |
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P, 5′-CCGGCTCTCCTCGCCCTCCA-3′ |