Table 1. Intron-skipping primers used for real-time PCR, their fixed threshold Ct values and expression levels in different breast tissue.
Gene | Gene expression assay ID | Threshold (Ct) value | Expression levels in M | Expression levels in NM | Expression levels in N | P-values |
---|---|---|---|---|---|---|
SRC | Hs00178494_m1 | 0.28263707 | 9041 | 9252 | 9493 | 0.976 |
LYN | Hs00176719_m1 | 0.34538345 | 7233 | 8922 | 10 521 | 0.076 |
LCK | Hs00178427_m1 | 0.26297827 | 655 | 217 | 255 | <0.001 |
FYN | Hs00176628_m1 | 0.27740355 | 2245 | 5293 | 25 484 | <0.001 |
FGR | Hs00178340_m1 | 0.22993330 | 1144 | 1275 | 1072 | 0.943 |
HCK | Hs00176654_m1 | 0.37934458 | 1815 | 2673 | 1712 | 0.070 |
BLK | Hs00176441_m1 | 0.23531063 | 208 | 161 | 234 | 0.114 |
YES | Hs00736972_m1 | 0.14854589 | 2142 | 499 | 3312 | <0.001 |
HPRT | 4310890E | 0.25742040 | NA | NA | NA | NA |
Abbreviations: M=invasive breast cancer; N=normal tissue; NA=not applicable; NM=non-malignant.
The table reveals details of each individual Src kinase family member and the housekeeping gene HPRT. Expression levels of Src kinase family members in different breast tissue types are stated as medians. P-values express alterations of expression in the different breast tissue types (Kruskal–Wallis test). Bold typeface is used to highlight significant P-values.