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. 2010 Aug 17;103(6):899–909. doi: 10.1038/sj.bjc.6605829

Table 1. Intron-skipping primers used for real-time PCR, their fixed threshold Ct values and expression levels in different breast tissue.

Gene Gene expression assay ID Threshold (Ct) value Expression levels in M Expression levels in NM Expression levels in N P-values
SRC Hs00178494_m1 0.28263707 9041 9252 9493 0.976
LYN Hs00176719_m1 0.34538345 7233 8922 10 521 0.076
LCK Hs00178427_m1 0.26297827 655 217 255 <0.001
FYN Hs00176628_m1 0.27740355 2245 5293 25 484 <0.001
FGR Hs00178340_m1 0.22993330 1144 1275 1072 0.943
HCK Hs00176654_m1 0.37934458 1815 2673 1712 0.070
BLK Hs00176441_m1 0.23531063 208 161 234 0.114
YES Hs00736972_m1 0.14854589 2142 499 3312 <0.001
HPRT 4310890E 0.25742040 NA NA NA NA

Abbreviations: M=invasive breast cancer; N=normal tissue; NA=not applicable; NM=non-malignant.

The table reveals details of each individual Src kinase family member and the housekeeping gene HPRT. Expression levels of Src kinase family members in different breast tissue types are stated as medians. P-values express alterations of expression in the different breast tissue types (Kruskal–Wallis test). Bold typeface is used to highlight significant P-values.