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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Invest. 2010 Nov;28(9):885–895. doi: 10.3109/07357907.2010.512816

Figure 2.

Figure 2

APE1/Ref-1 knockdown via siRNA results in a dose- and time-dependent decrease in APE1/Ref-1 protein in the mitochondria and nuclei. (A) Western blot of Panc-1 and PaCa-2 cells after transfection with APE1/Ref-1 siRNA. siRNA dose course on Day 3; oligofectamine (OF), scrambled (SC) and APE1/Ref-1 siRNA (Si). (B) Time course with siRNA 25 nM. (C) Representative Western blot probed for APE1/Ref-1 from mitochondria of PaCa-2 cells with quantitation (right). WCE = whole cell extract, Nuc/Cyto = Nuclei + cytoplasm, Mito = purified mitochondria.