Figure 7.

FoxO3a siRNA decreases serum-deprivated apoptosis. Cells were transfected with a control siRNA or FoxO3a siRNA. (A) FoxO3a expression was assessed by Western blotting using anti-FoxO3a Ab. (B) Control or FoxO3a siRNA cells were changed into serum-free medium, After 9 h, cells were incubated with 3 µM CpG ODN for 6 h, and FLIP mRNA levels were assessed by real-time RT-PCR. (C) siRNA-treated cells were changed with serum free media and treated with 3 µM CpG ODN for 12 h, cells were stained with annexin-V FITC, and analyzed for the induction of apoptosis by flow cytometry. Each data point represents the mean ± S.D. of four independent experiments (^*, P < 0.05, and ^**, P < 0.01).