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. 2010 Sep;131(1):40–49. doi: 10.1111/j.1365-2567.2010.03270.x

Figure 5.

Figure 5

Toll-like receptor-3 (TLR-3) activation up-regulates macrophage inflammatory protein 1-α/β (MIP-1α/β) expression. (a) MIP1-α/β messenger RNA (mRNA) expression. Seven-day-cultured macrophages were treated with or without poly I:C at the indicated concentrations for 12 hr. Total RNA extracted from cells was then subjected to the real-time polymerase chain reaction for the mRNA levels of MIP1-α, MIP1-β and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The data are expressed as MIP1-α or MIP1-β mRNA levels relative (fold) to the control (without poly I:C treatment, which is defined as 1). (b) MIP1-α/β protein expression. Seven day-cultured macrophages were treated with or without poly I:C at the indicated concentrations for 24 hr. Supernatants were then collected from cell cultures for the protein levels of MIP1-α/β. The results shown are the mean ± SD of triplicate wells, representing three independent experiments (**P < 0.01; *P < 0.05).