Table 1.

Primer sets for real-time reverse transcription–polymerase chain reaction

Primer	Accession no.	Orientation	Sequences
GAPDH	NM_002046	Sense:	5′-GGTGGTCTCCTCTGACTTCAACA-3′
		Antisense:	5′-GTTGCTGTAGCCAAATTCGTTGT-3′
IFN-α	NM_002175	Sense:	5′-TTTCTCCTGCCTGAAGGACAG-3′
		Antisense:	5′-GCTCATGATTTCTGCTCTGACA-3′
IFN-β	NM_002176	Sense:	5′-GCCGCATTGACCATCTATGAGA-3′
		Antisense:	5′-GAGATCTTCAGTTTCGGAGGTAAC-3′
IRF-1	NM_002198	Sense:	5′-TGAAGCTACAACAGATGAGG-3′
		Antisense:	5′-AGTAGGTACCCCTTCCCATC-3′
IRF-3	NM_001571	Sense:	5′-ACCAGCCGTGGACCAAGAG-3′
		Antisense:	5′-TACCAAGGCCCTGAGGCAC-3′
IRF-5	NM_001098631	Sense:	5′-AAGCCGATCCGGCCAA-3′
		Antisense:	5′-GGAAGTCCCGGCTCTTGTTAA-3′
IRF-7	NM_001572	Sense:	5′-TGGTCCTGGTGAAGCTGGAA-3′
		Antisense:	5′-GATGTCGTCATAGAGGCTGTTGG-3′
IRF-9	NM_006084	Sense:	5′-GCATCAGGCAGGGCACGCTGCACC-3′
		Antisense:	5′-GCCTGCATGTTTCCAGGGAATCCG-3′
TLR-1	NM_003263	Sense:	5′-GCCTATATGCAAAGAGTTTGGC-3′
		Antisense:	5′-CTCTCCTAAGACCAGCAAGACC-3′
TLR-2	NM_003264	Sense:	5′-GGCTTCTCTGTCTTGTGACC-3′
		Antisense:	5′-GGGCTTGAACCAGGAAGACG-3′
TLR-3	NM_003265	Sense:	5′-AGCCACCTGAAGTTGACTCAGG-3′
		Antisense:	5′-CAGTCAAATTCGTGCAGAAGGC-3′
TLR-4	NM_138554	Sense:	5′-CAGAGTTTCCTGCAATGGATCA-3′
		Antisense:	5′-GCTTATCTGAAGGTGTTGCACAT-3′
TLR-5	NM_003268	Sense:	5′-AGCCATCTGACTGCATTAAGG-3′
		Antisense:	5′-GACTTCCTCTTCATCACAACC-3′
TLR-6	NM_006068	Sense:	5′-ATTGAAAGCATTCGTGAAGAAG-3′
		Antisense:	5′-ACGGTGTACAAAGCTGTCTGTG-3′
TLR-7	NM_016562	Sense:	5′-AAAATGGTGTTTCCAATGTGG-3′
		Antisense:	5′-GGCAGAGTTTTAGGAAACCATC-3′
TLR-8	NM_138636	Sense:	5′-TTATGTGTTCCAGGAACTCAGAGAA-3′
		Antisense:	5′-TAATACCCAAGTTGATAGTCGATAAGTTTG-3′
TLR-9	NM_017442	Sense:	5′-TACCAACATCCTGATGCTAGACTC-3′
		Antisense:	5′-TAGGACAACAGCAGATACTCCAGG-3′
TLR-10	NM_001017388	Sense:	5′-GGCCAGAAACTGTGGTCAAT-3′
		Antisense:	5′-AAATGACTGCATCCAGGGAG-3′
MIP1-α	NM_002983	Sense:	5′-GCTGACTACTTTGAGACGAGC-3′
		Antisense:	5′-CCAGTCCATAGAAGAGGTAGC-3′
MIP1-β	NM_002984	Sense:	5′-CCAAACCAAAAGAAGCAAGC-3′
		Antisense:	5′-AGAAACAGTGACAGTGGACC-3′
A3G	NM_021822	Sense:	5′-TCAGAGGACGGCATGAGACTTAC-3′
		Antisense:	5′-AGCAGGACCCAGGTGTCATTG-3′
MxA	M_30817	Sense:	5′-GCCGGCTGTGGATATGCTA-3′
		Antisense:	5′-TTTATCGAAACATCTGTGAAAGCAA-3′
ISG56	X_03557	Sense:	5′-TTCGGAGAAAGGCATTAGA-3′
		Antisense:	5′-TCCAGGGCTTCATTCATAT-3′
OSA-1	NM_016816	Sense:	5′-AGAAGGCAGCTCACGAAACC-3′
		Antisense:	5′-CCACCACCCAAGTTTCCTGTA-3′
PKR	NM_003690	Sense:	5′-AGAGTAACCGTTGGTGACATAACCT-3′
		Antisense:	5′-GCAGCCTCTGCAGCTCTATGTT-3′
Tetherin	BC_033873	Sense:	5′-AAGAAAGTGGAGGAGCTTGAGG-3′
		Antisense:	5′-CCTGGTTTTCTCTTCTCAGTCG-3′