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. 2010 Sep;131(1):59–66. doi: 10.1111/j.1365-2567.2010.03274.x

Figure 4.

Figure 4

Effect of seladin-1 small interfering (si) RNA on lipopolysaccharide (LPS) -induced p38 activation. RAW 264.7 cells were transfected with seladin-1 siRNA or negative control siRNA for 48 hr and stimulated with LPS (100 ng/ml) for 1 hr. The expression of seladin-1 protein (a) and phosphorylated p38 (pp38), pERK1/2, pSAPK/JNK (b) were determined with immunoblotting. (c) The transfected cells were stimulated with LPS (100 ng/ml) for 30 min. The binding of the activated p65 nuclear factor-κB (NF-κB) subunit to an NF-κB consensus sequence was determined with the NF-κB DNA binding kit. (d) The transfected cells were stimulated with LPS (100 ng/ml) for 60 and 120 min. The expression of p53 protein was determined with immunoblotting. Relative quantification of immunoblots was analysed by densitometry and the result is shown as the mean of three experiments with SD (b,d).