Figure 6.

Determination of regulatory T cells based on marker analysis. (a) CD4⁺ CD8^– (left), CD4⁺ CD8⁺ (middle panel) and CD8⁺ CD4⁻ (right) T cells were segregated based on CD25 expression and FoxP3 analysis was performed as shown in the supplementary Fig. S2. Note that some CD8⁺ (CD4⁻) CD25^bright T-cells showed low FoxP3 expression in non-human primates. (b) Interleukin-7 receptor α (IL-7Rα) -expressing T cells in CD4⁺ CD8⁻ regulatory T cells (Tregs) based on FoxP3 analysis. Higher percentage of IL-7Rα⁺ T cells in Foxp3⁻ CD25^bright Tregs in peripheral blood mononuclear cells from humans than in those from non-human primates. **P <0·001, *P <0·05 (Mann–Whitney U-test).