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. 2010 Nov;177(5):2357–2365. doi: 10.2353/ajpath.2010.091131

Figure 6.

Figure 6

HHcy reduces phosphorylation of Akt (p-Act) in liver via the cAMP/CREB pathway. Mice were sacrificed at various times after PH. Liver removed at the time of PH was used as the baseline (zero time). A: Total proteins were extracted from livers and analyzed by Western blotting with antibodies against p-Akt (Ser473) (n = 5 per group at each time point). Representative Western blots are shown. B: Hepatic cAMP levels were measured using ELISA. Results are expressed as the mean ± SD (n = 5 per group at each time point). *P < 0.05 versus control (CTR). C: The levels of phosphorylated CREB (p-CREB) were measured using Western blotting analysis (n = 5 per group at each time point). Representative Western blots are shown. D: Primary cultured hepatocytes were preincubated with 5 μmol/L H89 or 100 μmol/L adenylyl cyclase toxin (ACT) for 30 minutes. After incubation with homocysteine for 3 hours, total proteins were extracted from livers and analyzed by Western blotting with antibodies against p-Akt (Ser473) (n = 4). Representative Western blots are shown.