Identification of the transcription
start sites in the rpoH gene in Y.frederiksenii.
(A) Major products from primer extension experiments
are indicated by numbers and black arrows (P1, P2 and P3). Lane
1, cells grown at 30°C; lane 2, cells
grown at 30°C and exposed to 43°C
for 5 min; lane 3, cells grown at 30°C and
exposed to 43°C for 10 min. (B)
Sequence of the regulatory region of the Y.frederiksenii
rpoH gene. Vertical arrows, transcription start sites. Potential –10
and –35 sequences of the promoters are underlined. Opposite
arrows underline the transcriptional terminator of ftsX.