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Effect of the nAChRα−7, α-bungarotoxin, on cotinine-induced PROKR1 expression in OE-E6/E7 cells. OE-E6/E7 cells were treated with 400 ng/ml cotinine or pretreated for 30 minutes with the nAChRα−7 antagonist, α-bungarotoxin, followed by 400 ng/ml cotinine. Cells were treated with cotinine for 8 hours for mRNA analysis (A) and for 9 hours for protein analysis (B). *P < 0.05.
