Figure 2.

The rate of monocyte efflux is markedly reduced in integrin α1-null Alport (DKO) mice relative to Alport mice. Animals were injected with Alexa-conjugated dextrans and harvested three days later at the indicated ages. The number of red (Alexa 568-positive)/green (CD11b-positive) dual-positive cells were quantified blinded with image pro plus software. Ten fields per section were quantified for three sections in triplicate experiments. Numbers represent the mean and standard deviations. The slopes of the best-fit curves are provided, showing a much greater slope for Alport mice compared to integrin α1-null Alport mice, consistent with a much slower rate of α1β1 integrin-positive monocyte efflux in the latter.