Figure 6.

Treatment of Alport mice with Ab2 markedly attenuates the efflux of monocytes into the interstitium of Alport kidneys. Alport mice were given bi-weekly intravenous injections of 25 μg Ab2 beginning at four weeks of age. Control mice were given the same regimen using isotype matched nonreactive antibodies (IgM). Three days before sacrifice, mice were injected with Alexa 568-conjugated dextrans. At seven weeks of age, kidneys were harvested and cryosections either visualized directly for newly effluxed monocytes (A and C) or immunostained for total monocyte accumulation using anti-CD11b antibodies (B and D). A and B show untreated Alport mice. C and D show Alport mice given the Ab2 injections. The fields shown are representative of 10 independent animals.