Skip to main content
. 2010 Nov;177(5):2549–2562. doi: 10.2353/ajpath.2010.100265

Figure 6.

Figure 6

CX3CL1-CX3CR1 signaling inhibits microglial phagocytosis. Cx3cr1+/+ (A and B) and Cx3cr1−/− (C and D) primary microglia were incubated in the absence (A and C) or presence (B and D) of CX3CL1 (100 nmol/L) overnight, and fluorescent microspheres were added for 90 minutes. The cells were fixed and stained with antibody against Iba1 and visualized with Alexa 648-conjugated secondary antibody (pseudo-colored green; A–D). Single-plane confocal images are shown. CX3CL1-treated Cx3cr1+/+ microglia exhibited reduced numbers of phagocytosed microspheres per cell when compared with untreated Cx3cr1+/+ and CX3CL1-treated Cx3cr1−/− microglia (E; *P < 0.05). n = 3 for each group. Scale bar = 15 μm.