Figure 3.

Inhibition of tissue-type plasminogen activator does not mediate neuroserpin-induced hypoxic tolerance in hippocampal neurons. Mean neuronal survival in hippocampal neurons cultured from Wt or tPA-deficient (tPA^−/−) mice and treated with neuroserpin 800 nmol/L either 24 hours prior to (A) or immediately before (B) exposure to lethal hypoxia (55 minutes of OGD conditions). Lines denote SD; n = 15 for each observation. Ns, neuroserpin; PREC, preconditioning. It denotes the moment when cells were treated with Ns.