Figure 5.

Fetuin-A-mediated inactivation of ARF-P19 and degradation of P53. Equal amounts of detergent-solubilized proteins of mammary tumors from the fetuin-A wild-type and null mice prepared as described in Materials and Methods were separated on a 4 to 12% SDS gel and transferred to nitrocellulose membranes, and the membranes were probed with antibodies to p53 (A) or p19ARF (C). Actin was used as a loading control. Note that the exposure of membranes probed with anti-p53 antibody was performed overnight at room temperature. Ub-p53, ubiquitinylated p53. B: Densitometric analysis of the p53 and Ub-p53 bands of whole tissue lysates. Bars represent means and SD of densitometric ratios relative to Fet^+/+ from blots of two representative experiments.