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HPLC traces of (A) Compound 4 in a RPMI medium; (B) degradation profile of compound 4 after 5 minutes of incubation at 37°C in RPMI containing glutathione medium (3 mM); (C) after 15 minutes; (D) after 60 minutes. The values shown over each peak are times of elution. [HPLC conditions: Beckman (250Z4 mm) RP-C18 (5 μm) column; solvent A :(1% TFA) H2O, solvent B: (1% TFA) CH3CN; A/B from 0/100 to 100/0 in 35 minutes; flow rate: 1 mL/min; 214 nm].
