FIG. 5.

Cellular uptake of the fluo-PNATAR-TPP conjugate 3 by flow cytometry analysis: CEM cells were incubated with the fluorescein-labeled conjugate for 6 hours at 37°C. After washing thoroughly with phosphate-buffered saline solution, the cells were resuspended in RPMI media containing 2% FCS. Analysis was done in presence of propidium iodide. Panel A represents cells grown in the absence of DMSO. R1 represents the field of viable cells; panels B and C represent cell grown in the absence of DMSO and in the presence of 1% DMSO, respectively, excluding approximately 4% propidium iodide positive dead cells; panel D shows flow cytometry data of the uptake of conjugate 3 after 6 hours of incubation in presence of 1% DMSO (blue peak), showing 43% uptake as compared to control (green peak).