FIG. 4.

cDNA gene expression profiles in VA13 cells at different densities. Autoradiograms of microarrays hybridized to RNA isolated from (A) sparse and (B) dense cells, respectively. The direction of orientation is indicated by arrow and the position numbers of genes on the array coincides with the numbers listed in Table 2. Phase contrast images corresponding to sparse and dense populations of VA13 are shown on left panels, respectively, next to autoradiograms. Location of hybridization signals for mismatch repair genes, MSH2 and MSH6, is shown in red circles. (C) Immunodetection of MSH2 in VA13 cells at different plating densities: 10% (Lane 1), 20% (Lane 2), 40% (Lane 3), 60% (Lane 4), 80% (Lane 5), and 100% (Lane 6). For each sample, 5 μg of nuclear proteins were resolved on SDS–PAGE gel, and MSH2 protein was detected with monoclonal antihuman MSH2 antibodies as described in Material and Methods.