Conditional deletion of Smo does not change the number of astrocytes. A, B, Bright-field immunostaining for S100β in the cortex shows no difference in the number of astrocytes in mGFAP-Smo CKO animals (B) compared to littermate controls (A). C, Quantification of the number of cortical astrocytes in mGFAP-Smo CKO animals and littermate controls (mean ± SEM; control, n = 4, mGFAP-Smo CKO, n = 3, unpaired t test). Scale bar, 50 μm.