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. Author manuscript; available in PMC: 2010 Oct 31.
Published in final edited form as: Genesis. 2007 Oct;45(10):647–652. doi: 10.1002/dvg.20341

Figure 4. Quantitative RT-PCR (qRT-PCR) measurements of the relative amounts of GFP and nucleostemin RNAs in the adult testes of transgenic mice.

Figure 4

The RNA expression levels of GFP, nucleostemin, and RNA polymerase II were measured by qRT-PCR using 1st stranded cDNAs synthesized from the adult testes of wild-type and transgenic (NS+/+; TG+/−) mice. (a) After normalization to the amount of RNA polymerase II, the relative levels of the GFP RNA are 1.3, 1.0, 4.4, 4.4, and 5.9 in the NSiGFP#1, NSiGFP#5, NSiGFP#17, NSmGFP#1, and NSmGFP#14 testes. The expression levels of the nucleostemin RNA transcript of these five transgenic lines relative to the wild-type mouse (FVB) were determined by qRT-PCR assays using primers that recognize the middle portion (b) or the 3’ end (c) of nucleostemin. The expression levels of the nucleostemin RNA were determined in the wild-type (C57/B6) and the NS+/− testis to provide a reference for the qPCR measurment (b2). (d) A schematic diagram of the bicistronic transgenic RNA and the regions detected by the qRT-PCR assay in Fig. 4a, 4b, and 4c (thick lines). The 13th intron with an engineered loxP site (arrowhead) is shown at the bottom.