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. Author manuscript; available in PMC: 2010 Oct 31.
Published in final edited form as: Mol Cell Proteomics. 2007 Sep 11;6(12):2058–2071. doi: 10.1074/mcp.M700123-MCP200

Figure 7. BR regulates BiP2 phosphorylation.

Figure 7

BR treatment and the bzr1-1D mutation affect phosphorylation of BiP2. From top panel to bottom in: Overlaid 2-D DIGE images of BR treated (Cy5, red) compared with untreated (Cy3, green) det2 samples, 2-D DIGE image of transgenic line expressing mBZR1-CFP (Cy5, red) compared with wild type (Cy3), and Pro-Q Diamond stain and Cy5 images from the same 2-DE gel, and the table showing the quantified data. A higher ratio of Pro-Q diamond to Cy5 signal indicates increased phosphorylation. The Pro-Q/Cy5 ratio was normalized to spot 3.