Table 1.
BR-regulated proteins. Average protein level ratios of BR-treated/untreated (positive) or BR-untreated/BR-treated (negative) and the t-test p-values are calculated from three repeat experiments of the 24 hr time point.
| Spot | Gene Locus | Protein name | Mascot Score |
6-hr ratio |
6-hr p-value |
12-hr ratio |
12-hr p-value |
24-hr ratio |
24-hr p-value |
|---|---|---|---|---|---|---|---|---|---|
| Secretion and membrane trafficking | |||||||||
| 311 | At1g22530 a | Sec14 cytosolic factor (PATL2) | 486 | 2.16 | 3.0E-2 | 1.72 | 1.4E-2 | 2.69 | 1.5E-2 |
| 317 | At1g22530 a | Sec14 cytosolic factor (PATL2) | 407 | 2.13 | 2.1E-3 | 2.07 | 6.3E-4 | 2.78 | 6.6E-3 |
| 321 | At1g22530 a | Sec14 cytosolic factor (PATL2) | 473 | 1.88 | 3.9E-2 | 2.04 | 1.5E-3 | 2.87 | 8.8E-3 |
| 742 | At1g72150 | Sec14 cytosolic factor (PATL1) | 336 | 1.45 | 6.0E-3 | 1.81 | 6.5E-2 | 3.22 | 8.2E-6 |
| 745 | At1g72150 | Sec14 cytosolic factor (PATL1) | 967 | 1.97 | 1.8E-2 | 2.19 | 2.0E-4 | 3.51 | 3.0E-5 |
| 801 | At1g30690 a | Sec14 cytosolic factor (PATL4) | 364 | 2.16 | 3.0E-2 | 1.21 | 2.3E-1 | 1.86 | 5.0E-3 |
| Signal protein | |||||||||
| 2770 | At2g41100 b | calmodulin-related protein 3 (TCH3) | 589 | 1.00 | 1.0E+0 | 1.05 | 5.0E-2 | 1.59 | 7.7E-3 |
| 3108 | At5g10450 | 14-3-3 protein GF14 lambda (GRF6) | 527 | 1.06 | 1.4E-1 | 1.09 | 1.0E-2 | 1.27 | 6.6E-4 |
| 3115 | At5g65430 | 14-3-3 protein GF14 kappa (GRF8) | 118 | 1.05 | 5.6E-3 | 1.11 | 1.2E-1 | 1.38 | 3.1E-5 |
| 3299 | At2g41090 a,b | calmodulin-like protein (CABP-22) | 163 | 1.01 | 7.2E-1 | 1.78 | 2.7E-2 | 5.33 | 5.6E-4 |
| Hormone and vitamin biosynthesis | |||||||||
| 2168 | At1g76680 a | 12-oxophytodienoate reductase (OPR1) | 404 | −1.03 | 5.8E-1 | 1.35 | 6.3E-2 | 1.52 | 4.6E-3 |
| 2295 | At1g62380 | ACC oxidase (ACO2 ) | 356 | −1.02 | 9.0E-2 | −1.13 | 3.5E-4 | −1.55 | 6.3E-4 |
| 2296 | At1g62380 | ACC oxidase (ACO2 ) | 491 | −1.04 | 1.1E-2 | 1.18 | 2.9E-1 | −1.32 | 3.0E-2 |
| 3603 | At3g25770 | allene oxide cyclase (AOC2) | 112 | 1.04 | 7.3E-1 | 1.15 | 1.2E-3 | 1.67 | 1.6E-3 |
| 2898 | At5g54770 | thiazole biosynthetic enzyme (THI1) | 431 | 1.01 | 9.2E-1 | −1.25 | 6.2E-2 | −3.16 | 1.9E-3 |
| 2957 | At5g54770 | thiazole biosynthetic enzyme (THI1) | 390 | 1.02 | 7.3E-1 | −1.44 | 1.4E-2 | −3.37 | 2.0E-2 |
| Cytoskeleton | |||||||||
| 1734 | At5g44340 b | tubulin beta-4 chain (TUB4) | 490 | 1.14 | 2.9E-1 | 1.05 | 1.7E-2 | 1.42 | 2.5E-2 |
| 1792 | At4g14960 b | tubulin alpha-6 chain (TUA6) | 1016 | 1.16 | 5.5E-2 | 1.14 | 1.2E-1 | 1.39 | 8.5E-3 |
| 2075 | At3g18780 | actin2 (ACT2) | 1248 | 1.08 | 8.3E-3 | 1.13 | 1.1E-2 | 1.34 | 1.3E-2 |
| Heat shock proteins (chaperones) | |||||||||
| 819 | At4g24190 | SHEPHERD (GRP94) | 1271 | 1.14 | 3.4E-1 | 1.19 | 4.8E-2 | 1.36 | 2.1E-3 |
| 1122 | At5g42020 | luminal binding protein 2 (BiP-2) | 1956 | 1.16 | 4.9E-2 | 1.24 | 1.1E-2 | 1.58 | 3.0E-3 |
| 1150 | At5g02490 a | heat shock cognate (HSC70-2) | 962 | −1.02 | 7.0E-1 | 1.10 | 4.3E-3 | 1.69 | 1.0E-2 |
| 1214 | At3g12580 a | heat shock protein 70 | 880 | 1.12 | 7.7E-2 | 1.29 | 4.8E-2 | 1.47 | 9.9E-3 |
| Redox related proteins | |||||||||
| 1941 | At3g09940 | monodehydroascorbate reductase MDAR3 |
377 | 1.03 | 2.6E-1 | 1.02 | 3.7E-1 | 1.59 | 4.0E-3 |
| 2294 | At1g76180 | dehydrin ERD14 | 188 | −1.13 | 9.0E-2 | 1.09 | 1.4E-1 | −1.34 | 2.2E-3 |
| 2353 | At2g47470 | thioredoxin family protein | 454 | 1.09 | 2.6E-1 | 1.18 | 2.9E-1 | 1.30 | 3.2E-2 |
| 2497 | At1g54040 | kelch repeat-containing protein | 372 | −1.02 | 3.5E-1 | 1.70 | 1.1E-2 | 1.53 | 1.3E-2 |
| 2506 | At1g54040 | kelch repeat-containing protein | 908 | 1.01 | 9.6E-1 | 1.03 | 9.1E-1 | 1.87 | 2.1E-6 |
| 2635 | At4g12720 a | MutT/nudix family protein (GFG1) | 296 | 1.41 | 1.7E-2 | 1.86 | 1.9E-3 | 2.89 | 3.2E-2 |
| 3034 | At1g10370 | glutathione S-transferase (GSTU17) | 164 | 1.07 | 4.4E-1 | −1.42 | 1.4E-2 | 1.67 | 6.4E-6 |
| 3093 | At4g30530 | defense-related protein | 376 | 1.10 | 2.3E-1 | 1.18 | 1.8E-2 | 1.59 | 1.2E-5 |
| 3129 | At5g20630 | germin-like protein (GER3) | 174 | 1.08 | 3.0E-2 | 1.22 | 2.4E-2 | 1.48 | 1.4E-2 |
| 3279 | At4g02520 b | glutathione S-transferase (GSTF2) | 211 | 1.66 | 8.0E-2 | −1.03 | 7.9E-1 | 1.70 | 4.0E-4 |
| 3286 | At2g30860 b | glutathione S-transferase (GSTF9) | 234 | −1.10 | 2.2E-1 | 1.04 | 6.3E-1 | 1.34 | 5.7E-5 |
| 3291 | At4g02520 b | glutathione S-transferase(GSTF2) | 929 | −1.05 | 5.4E-1 | −1.05 | 5.3E-1 | 1.33 | 4.8E-3 |
| 3308 | At1g02920 a,b | glutathione S-transferase (GSTF7) | 356 | −1.04 | 5.1E-1 | −1.01 | 8.8E-1 | 1.43 | 2.0E-4 |
| 3344 | At2g47730 b | glutathione S-transferase 6 (GST6) | 328 | −1.02 | 7.4E-1 | 1.15 | 3.1E-1 | 1.52 | 2.9E-3 |
| 4536 | At1g02930b | glutathione S-transferase 1 (GST1) | 177 | −1.07 | 3.7E-1 | 1.30 | 2.9E-2 | 1.88 | 9.5E-4 |
| Metabolism | |||||||||
| 953 | At3g46970 | alpha-glucan phosphorylase (PHS2) | 78 | 1.12 | 1.1E-1 | 1.13 | 8.3E-3 | 1.34 | 4.3E-5 |
| 1433 | At5g11670 | malate oxidoreductase (NADP-ME2) | 550 | 1.03 | 1.2E-1 | 1.05 | 1.9E-1 | 1.65 | 2.6E-3 |
| 1769 | At3g23810a | S-adenosyl-L-homocysteine hydrolase | 652 | 1.11 | 1.6E-1 | 1.21 | 4.9E-2 | 1.63 | 2.6E-4 |
| 2057 | At3g17390 b | S-adenosylmethionine synthetase | 826 | 1.13 | 1.1E-3 | 1.17 | 1.6E-1 | 1.46 | 5.4E-5 |
| 2062 | At1g08200b | putative UDP-D-apiose synthetase | 662 | 1.15 | 5.8E-2 | 1.21 | 1.8E-4 | 1.39 | 1.6E-3 |
| 2088 | At4g23600 | coronatine-responsive tyrosine aminotransferase |
1265 | 1.04 | 4.6E-1 | 1.09 | 1.4E-1 | 1.46 | 3.0E-5 |
| 2239 | At5g37600 | glutamine synthetase | 460 | 1.09 | 3.8E-2 | 1.29 | 3.1E-2 | 1.89 | 3.1E-4 |
| 2362 | At3g44860 | SAM:carboxyl methyltransferase | 218 | 1.07 | 7.2E-1 | 1.02 | 8.5E-1 | 1.59 | 9.3E-3 |
| Others | |||||||||
| 1937 | At1g57720 | elongation factor 1B–gamma | 612 | −1.01 | 7.8E-1 | −1.02 | 6.5E-1 | 3.10 | 2.4E-2 |
| 2866 | At3g53460 | 29 kDa chloroplast ribonucleoprotein | 603 | 1.02 | 8.4E-1 | 1.03 | 6.1E-1 | −1.24 | 6.1E-4 |
| 2880 | At3g53460 | 29 kDa chloroplast ribonucleoprotein | 613 | −1.01 | 8.1E-1 | 1.05 | 4.0E-2 | −1.34 | 8.3E-3 |
| 3177 | At1g50670 | OTU-like cysteine protease | 83 | 1.66 | 8.0E-2 | 1.47 | 2.8E-1 | 2.22 | 2.0E-3 |