Figure 4.

α₄β₁ integrin and Lu/BCAM are involved in RBC-PBMC aggregation. Inhibition assays using VCAM-1-Fc (A) or anti-β1 antibody (B). *P=0.03, Wilcoxon's matched-pairs test. (C) The dot plot representation in the left panel shows gated events corresponding to SS RBC, PBMC and aggregates. Each of these three populations was gated and analyzed for Lu/BCAM expression, as shown in the middle panel. Lu/BCAM expression in aggregates is magnified in the right panel. Bold horizontal lines represent Lu/BCAM-positive events. (D) Percentage of Lu/BCAM-positive events in the RBC population (▴) and in aggregates (▾) of five SS patients. Horizontal lines indicate medians.