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. 2010 Jul 15;95(11):1889–1896. doi: 10.3324/haematol.2010.027821

Figure 2.

Figure 2.

When CLL cells are cultured in the presence of CD40L, lycorine induces apoptosis in the presence of bezafibrate (BEZ)+MPA. CLL cells were cultured in a 10:1 ratio on stromal cells expressing CD40L and were treated with solvent control, or a dose titration of lycorine either with or without BEZ+MPA for 72 h prior to analysis of the binding of annexin V and exclusion of propidium iodide. (A) Representative plots (from six CLL samples) of solvent control, 0.625 μM and 10 μM lycorine with and without BEZ+MPA. The lower left (LL) quadrant shows viable cells, lower right (LR) indicates cells in early stages of apoptosis, upper right (UR) is indicative of late apoptosis. Total annexin V staining was calculated by adding the events in the LR with those in the UR. (B) Dose titration of lycorine (10 μM-0.625 μM) without (black circles) and with (open circles) BEZ+MPA. The plot shown represents total annexin V positivity of six CLL samples ±s.e.m.