Figure 1. AMD3100-induced PC mobilization is impaired in c-kit^w/w-v mice.

(A-B) WT and c-kit^W/W-V mice received subcutaneous injections of AMD3100 (5 mg/kg) or PBS; 2 hours later, the numbers of colony-forming PCs in the PB-MNC (A) and BM-MNC (B) populations were evaluated via the colony-forming assay. (C-F) In the BM clearance/repopulation assay, WT and c-kit^w/w-v mice were treated with AMD3100 for 2 hours to mobilize endogenous PCs from the BM, then 40×10⁶ eGFP-transgenic BM MNCs were injected intravenously to compete with the endogenous PCs for repopulation of the BM. Three hours later, BM MNCs were isolated from the recipients and analyzed via flow cytometry to determine the proportion of injected (eGFP⁺) cells in the total BM MNC population (C), the proportion of eGFP⁺ BM MNCs that were CXCR4⁺ (D), Lin⁺, or Lin⁻ (E), and the proportion of eGFP⁺Lin⁻ BM MNCs that were CXCR4⁺, Sca-1⁺CXCR4⁺ and c-kit⁺CXCR4⁺ (F). Values are mean ± SEM; n=10 per group.