Figure 1.

Growth factor receptor-bound protein 14 interacts with cyclic-nucleotide-gated channel alpha subunit in vivo and modulates its function. (A,B) Retinal lysates or solubilized ROS from dark- and light-adapted rats were immunoprecipitated with CNGA1 antibody and immunoblotted with Grb14 and CNGA1 antibodies. (C) CNGA1 was co-expressed with Grb14 in the HEK293T cells and channel activity in each case was measured as a function of Ca²⁺ influx in response to cGMP stimulation. The data are mean±s.d., n=3 independent transfections. Significance between CNGA1 and CNGA1+Grb14, ^*P<0.001. (D) Protein expression in cell lysates was determined by immunoblotting with Myc, CNGB1 and actin antibodies. cGMP, cyclic guanosine monophosphate; CNGA1, cyclic-nucleotide-gated channel alpha subunit; CNGB1, cyclic-nucleotide-gated channel beta subunit; Grb14, growth factor receptor-bound protein 14; IP, immunoprecipitated; ROS, rod outer segment.