Figure 1.

Cleavage-stage arrest of 3-phosphoinositide-dependent protein kinase 1 maternally mutant embryos. (A) Developmental arrest at the two-cell stage of EmPdk1^+/− embryos. EmPdk1^+/+ and EmPdk1^+/− embryos were collected on E1.5 and were cultured for 3 days. Scale bar, 50 μm. (B) The average numbers of two-cell embryos per Pdk1^loxP/loxP and Pdk1^loxP/loxP;Zp3-Cre female after mating. Numbers of mice used (n) are shown. (C) Percentages of EmPdk1^+/+ and EmPdk1^+/− embryos that were arrested at the two-cell stage. Numbers of embryos used (n) are shown. (D) Western blots showing the absence of PDK1 protein in two-cell EmPdk1^+/− embryos. β-Actin was used as an internal control. NS, not significant; PDK1, 3-phosphoinositide-dependent protein kinase 1.