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. 2010 Oct 8;11(11):876–882. doi: 10.1038/embor.2010.153

Figure 3.

Figure 3

Cyclin-dependent kinase 9 activity is essential for its activities in the replication stress response. (A,B) U2OS cells stably expressing an empty vector, wild-type FLAG–HA–CDK9 or FLAG–HA–CDK9 D167N were transfected with NT or CDK9 siRNA directed against the 3′-UTR of CDK9, treated with 3 mM HU or 15 μM APH for 20 h and released into nocodazole for 10 h. DNA content was analysed by flow cytometry. (B) The percentage (mean and s.d.) of cells that completed DNA synthesis in three replicate experiments is shown. (C) Western blot analysis demonstrating expression of fusion proteins and knockdown of endogenous CDK9. APH, aphidicolin; CDK9, cyclin-dependent kinase 9; HA, haemagglutinin; HU, hydroxyurea; NT, nontargeting; siRNA, small interfering RNA; UTR, untranslated region.