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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biomaterials. 2010 Aug 4;31(31):7883–7891. doi: 10.1016/j.biomaterials.2010.07.013

Figure 6.

Figure 6

Single plane confocal image of astroglial cells stained for anti-Kir4. Note lower immunosignal intensity indicating low expression level of Kir4.1 in plane silk coated cells (A) compared to silk+GUO plated cells (B). C-D) High magnification single plane confocal images of cells revealed that in silk plated cells Kir expression pattern was more mislocalized explaining absence of current upon this experimental condition. On the contrary higher and membrane polarized expression pattern of Kir4.1(arrows) can be observed in cells plated on silk film embedded with guanosine (D). E-F Graph plot of fluorescence intenstity (pixel) in μm spatial range analyzed from silk astrocyted (E) and silk+GUO cells (F). Note higher immunofluorescence specificity of Kir 4.1 immunosignal for silk+GUO cells (F). Scale bar is 50 μm. G) Secondary antibody specificity control. Single plane confocal image of immunofluorescent experiment performed without addition of primary-antibody of immunofluorescence. Note that no immunofluorescent signal was observed, supporting the specificity of the assay.