Table I.
Primers used in this study for PCR and nucleotide sequencing
| Gene target/amplicon size (bp) | Primer pair (forward/reverse) |
| General sequencing primers | |
| λNU region (600 bp) | Nu1-1: 5′-CGGGTTTTCGCTATTTATGA-3′ |
| Nu1-2: 5′-ACATGTCGGTTTTCCAGTTC-3′ | |
| λlom, ORF401-like coding sequence side tail fiber and INT genes (3929 bp) | LO-1: 5′-ATGGCAGTAAAGATTTCAGG-3′ |
| LO-2: 5′-ACAGCAGGCCACTCAATATC-3′ | |
| λb527 mutation (972 bp) | B5-1: 5′-GAATCAATTCCAATTACCTGAAGTC-3′ |
| B5-2: 5′-GTATCAAGGTATTTTATGCGC-3′ | |
| λN-O region (6551 bp) | NO-1 5′-TTTTCCCTTAATTTTCTGGC-3′ |
| NO-2: 5′-TTACCGGACCAGAAGTTCTC-3′ | |
| E.coli lacZ (3341 bp) | LZ-1: 5′-GAATTCCGATTCATTAAT-3′ |
| LZ-2: 5′-GAATTCAAATAGTACATAATGG-3′ | |
| λTail region (582 bp) | TR-1: 5′-TATGTCCACAGCCCTGACG-3′ |
| TR-2: 5′-CTCGTATCACATGGAAGG-3′ | |
| λReplication origin (ori) (149 bp) (RT-PCR) | ORI-1: 5′-GACCCAACTCGAAATCAAC-3′ |
| ORI-2: 5′-ATCTGCTCACGGTCAAAG-3′ | |
| (501 bp) Ori Southern hybridization | ORI-S1: 5′-GGACAGGAGCGTAATGTGGCA-3′ |
| ORI-S2: 5′-AATTGCAGCATCCGGTTTCAC-3′ | |
| (812 bp) 18S Southern hybridization | 18SS-1: 5′-TCTTTCGAGGCCCTGTAAT-3′ |
| 18SS-2: 5′-ACCAACTAAGAACGGCCAT-3′ | |
| Mus musculus 18S rDNA gene (117 bp) (RT-PCR) | 18S-1: 5′-ACGGACAGGATTGACAGATTG-3′ |
| 18S-2: 5′-CCAGAGTCTCGTTCGTTATCG-3′ | |
| Mus musculus annexin V gene exon 4 (115 bp) (RT-PCR) | AN-1: 5′-GCATCCTGAACCTGTTGACATC-3′ |
| AN-2: 5′-ACACCCACTCCACCTTGAATG-3′ | |
| λ inverse primers | |
| (Position 340) | INV-1: 5′-CTTTTTGGCCTCTGTCGTT-3′ |
| (Position 97) | INV-2: 5′-TCCTTTCTTTTCAGAGGGTA-3′ |
| Genome walking λgt10-lacZ primers | |
| (Extending into 5′end, position 101) | GW-1 5′-CACCTGTCGTTTCCTTTCTTTTCAGAG- 3′ |
| (Extending into 5′end, position 44) | GW-2: 5′-GAAGAAGAACGGAAACGCCTTAAACC-3′ |
| (Extending to 3′ end, position 48195) | GW-3: 5′-AATTCCCGGACCCTTTTTGCTCAAGAGC-3′ |
| (Extending to 3′end, position 48475) | GW-4: 5′-GTCCTTTCCGGTGATCCGACAGGTTAC-3′ |
| Primers to scan for detection of transgene integration | |
| Biotinylated transgene primer | BIO-1 5′-CAGCATCCCTTTCGGCATACCATT-3′ |
| Degenerate primers | |
| FP1 | 5′-GACTCAGATATCGGCAGCGTGGTNNNNNNNGCGCT-3′ |
| FP2 | 5′-GACTCAGATATCGGCAGCGTGGTNNNNNNNGGCCT-3′ |
| FP3 | 5′-GACTCAGATATCGGCAGCGTGGTNNNNNNNGCGCA-3′ |
| FP4 | 5′-GACTCAGATATCGGCAGCGTGGTNNNNNNNGGCCA-3′ |
| Nested gene specific primers | |
| GSP1 | 5′-ACTATAGGGCACGCGTGGT-3′ |
| GSP2 | 5′-ACTTCCATTGTTCATTCCAC-3′ |