Fig. 3.

Microtubule disrupting agents suppresses the DNA damage-induced NF-κB binding activity. HeLa cells were treated with Adr (10 µg/mL) or Cpt (100 µM) for various times as indicated in the presence or absence of Col (A, 10 µM), Vin (B, 1 µM) and Noc (C, 0.5 µM). Nuclear extracts were prepared and 5 µg of nuclear extracts from each sample was used to analyze NF-κB binding activity by EMSA as described in Fig. 2B. As a control, 50 µg of the nuclear extracts were applied to SDS-PAGE for immunoblotting with anti-SP1 antibody.