Fig. 4.

Microtubule disrupting agents does not affect the DNA damage-induced IκB-α degradation and nuclear translocation of NF-κB subunit p65. HeLa cells were treated with Adr (10 µg/mL) or Cpt (100 µM) for various times as indicated in the presence or absence of Col (10 µM), Vin (1 µM) and Noc (0.5 µM). (A, B) Total cell extracts were prepared and applied to SDS-PAGE for immunoblotting with anti-IκB-α and anti-IKK-β antibodies. (C, D) Nuclear extracts were obtained as described Fig.1B, and the nuclear translocation of NF-κB subunit p65 was analyzed by immunoblotting with anti-p65 and anti-p84N5 antibodies from nuclear extracts (50 µg) from each sample.