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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Cycle. 2009 Mar 26;8(6):940–949. doi: 10.4161/cc.8.6.7948

Figure 1.

Figure 1

Reduced growth of CAML−/− MEFs. (A) Immunoblotting of lysates from CAML−/− and CAMLfl/fl MEFs. The CAML gene was inactivated in CAMLfl/fl MEFs by retroviral transduction of Cre recombinase. (B) Characterizing the growth feature of CAML−/− MEFs by direct cell count. Passage 4 (p4) CAML−/− and CAMLfl/fl MEFs were seeded in triplicates in culture medium with 10% FBS. Cell numbers were counted after overnight culture and this number was set as the initial number. After 24 h, the final cell numbers for each cell line were counted. Data is presented as the growth index (fold change = final cell count/initial number). Error bars indicate standard deviation. (C) CAML−/− and CAMLfl/fl MEFs stained for senescence associated β-galactosidase (SA-β-Gal) and photographed at 60× magnification. Cells from both early passage (p5) and late passage (p11) are shown. (D) Quantitation of SA-β-Gal-positive CAML−/− and CAMLfl/fl MEFs at p5 and p11.