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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Cycle. 2009 Mar 26;8(6):940–949. doi: 10.4161/cc.8.6.7948

Figure 2.

Figure 2

Loss of CAML causes a cut phenotype. (A) High incidence of anaphase failure in CAML−/− MEFs. Chromosomes were visualized by transducing MEFs with retrovirus encoding mRFP-H2B and followed by time-lapse microscopy. Time-lapse images showing a CAMLfl/fl MEF undergoing normal mitosis and two CAML−/− MEFs with distinct cut phenotypes. Scale bar = 10 µm. (B) Incidence of cut phenotypes in the indicated MEFs. (C) CAML−/− metaphase spread illustrating absence of diplochromosomes. (D) Topo IIα accumulates normally at inner centromeric regions of CAML−/− prometaphases. Monastrol-treated CAML−/− and CAMLfl/fl MEFs were immunostained for centromeres and Topo IIα, while DNA was stained with Hoechst.