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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Circ Res. 2010 Aug 12;107(8):10.1161/CIRCRESAHA.110.220764. doi: 10.1161/CIRCRESAHA.110.220764

Figure 2.

Figure 2

GATA-6 is required for pressure overload-induced cardiac hypertrophy. A, Western blot for GATA-4 and GATA-6 from cardiac nuclear protein extracts from sham and TAC operated wildtype mice. Lamin A/C was used as a loading control. B, Gel shift assay for total GATA binding activity in sham and TAC operated adult, wildtype mice from heart protein nuclear extracts. Arrow indicates GATA specific binding. C, VW/BW from sham and TAC-operated adult mice after 2 weeks of pressure overload. *P<0.05 vs sham, †P<0.05 vs βMHC-cre and Gata6fl/fl TAC. D. Cardiomyocyte cell surface area measured from histological sections. *P<0.05 vs sham; †P<0.05 vs βMHC-cre and Gata6fl/fl TAC. E, Cardiac fractional shortening percentage (FS%) from sham and TAC operated mice after 2 weeks of pressure overload, measured by M-mode echocardiography. *P<0.05 vs βMHC-cre and Gata6fl/fl TAC. Number of mice analyzed is shown in the bars. F, Taqman qPCR for expression of ANF mRNA in sham and TAC operated mice. G, Taqman qPCR for expression of αMHC mRNA in sham and TAC operated mice. *P<0.05 vs βMHC-cre and Gata6fl/fl sham, †P<0.05 vs βMHC-cre TAC. H, Taqman qPCR for expression of skeletal α-actin mRNA in sham and TAC operated mice. *P<0.05 vs βMHC-cre and Gata6fl/fl TAC.