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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Glia. 2010 May;58(7):839–847. doi: 10.1002/glia.20968

FIGURE 4.

FIGURE 4

Activation of NF-kB following exposure to intact S. pneumoniae is significantly lower in glia deficient in the expression of NOD2. Microglia (Upper immunoblot) or astrocytes (Lower immunoblot) (2 × 106 cells per well) from wild type (NOD2+/+) and NOD2 knockout (NOD2-/-) animals were untreated (0) or exposed to viable S. pneumoniae (MOI, of 25:1, 75:1, 250:1 bacteria to each glial cell). At 2 hrs following bacterial challenge nuclear protein isolates were prepared and assayed for the presence of NF-kB p65 (RelA) by immunoblot analysis. Fold increases in nuclear RelA levels following bacterial challenge were determined by densitometric analysis and are indicated below the bands. The immunoblots shown are representative of three separate experiments.